This is the old version of CHOPCHOP.
The new CHOPCHOP is available at

or paste input sequence

Target specific region of gene

Restrict targeting

Restriction enzymes

Method for determining off-targets in the genome:

5' requirements for gRNA:

PAM motif:

Distance between TALENs:

Size of TALENs:

Number of mismatches to search for when finding off-targets:

G repeat variable diresidue (RVD):

Product size:

Primer size:

Primer Tm:

Minimum distance from primer to target site:

Toggle advanced options