or paste input sequence

Target specific region of gene






Restrict targeting



Restriction enzymes


Method for determining off-targets in the genome:




5' requirements for gRNA:




PAM motif:




Distance between TALENs:

Size of TALENs:

Number of mismatches to search for when finding off-targets:

G repeat variable diresidue (RVD):



Product size:

Primer size:

Primer Tm:

Minimum distance from primer to target site:

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