CHOPCHOP is a web tool for selecting the optimum target sites for CRISPR/Cas9- or TALEN-directed mutagenesis.

CHOPCHOP can be run with as few as three basic input options, or with additional advanced parameters. The basic input comprises: (i) a gene name, genomic coordinates or a pasted sequence (including RefSeq and ENSEMBL gene IDs); (ii) a growing list of organisms and (iii) the choice between CRISPR/Cas9 or TALEN mode. The advanced options provide the user with more flexibility when choosing target sites.

If you use our tool please cite:

Tessa G. Montague; Jose M. Cruz; James A. Gagnon; George M. Church; Eivind Valen. (2014). CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing. Nucleic Acids Res. 42. W401-W407

This work was supported by a National Defense Science and Engineering Graduate (NDSEG) Fellowship; the American Cancer Society; the Human Frontier Science Program and the National Human Genome Research Institute (NHGRI) Center for Excellence in Genomics Science.

CHOPCHOP is for non-profit and academic use only. All commercial rights are reserved.